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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
14/09/2023 |
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Actualizado : |
14/09/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GIORELLO, F.M.; FARIAS, J.; BASILE, P.; BALMELLI, G.; DA SILVA, C.C. |
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Afiliación : |
FACUNDO M. GIORELLO, Espacio de Biología Vegetal del Noreste, Centro Universitario Regional Noreste, sede Tacuarembó, Universidad de la República, Tacuarembó, Uruguay; JOAQUINA FARIAS, Espacio de Biología Vegetal del Noreste, Centro Universitario Regional Noreste, sede Tacuarembó, Universidad de la República, Tacuarembó, Uruguay; PATRICIA BASILE, Espacio de Biología Vegetal del Noreste, Centro Universitario Regional Noreste, sede Tacuarembó, Universidad de la República, Tacuarembó, Urugu; GUSTAVO DANIEL BALMELLI HERNANDEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CECILIA CORINA DA SILVA, Espacio de Biología Vegetal del Noreste, Centro Universitario Regional Noreste, sede Tacuarembó, Universidad de la República, Tacuarembó, Uruguay. |
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Título : |
Evaluating the potential of XP-GWAS in Eucalyptus: Leaf heteroblasty as a case study. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Plant Gene. December 2023, Volume 36, article 100430. https://doi.org/10.1016/j.plgene.2023.100430 |
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ISSN : |
2352-4073 |
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DOI : |
10.1016/j.plgene.2023.100430 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 3 February 2023, Revised 24 July 2023, Accepted 11 August 2023, Available online 22 August 2023, Version of Record 31 August 2023. -- Correspondence: Giorello, F.M.; Espacio de Biología Vegetal del Noreste, Centro Universitario Regional Noreste, sede Tacuarembó, Universidad de la República, Tacuarembó, Uruguay; email:facundo.giorello@cut.edu.uy -- FUNDING: This work was supported by grant of the Ministerio de Educación y Cultura, Uruguay (Fondo Vaz Ferreira 173, DICyT, MEC). -- |
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Contenido : |
ABSTRACT.- Eucalyptus is one of the mainstays of the forest industry, contributing high-quality raw materials for pulp, paper, wood, and energy production. The typical approaches to reveal the genetic basis of important traits include classical Quantitative Trait Locus (QTL) mapping and Genome-Wide Association Studies (GWAS) approaches, but these are typically expensive and time-consuming. Here we evaluate the potential of Extreme-Phenotype GWAS (XP-GWAS) to identify candidate genes underlying a quantitative trait in Eucalyptus, using the timing of leaf heteroblasty as a case study. XP-GWAS involves genotyping pools of individuals grouped by extreme and opposed phenotypes from a population or a diversity panel and studying their allele frequency. Using a previous phenotyped trial of E. globulus, we sequenced pools of 50 individuals that notably differ in the onset of adult foliage. Since the genetic basis of heteroblasty is well understood, we first searched for previously identified genes. Secondly, we searched for new candidate genes and also evaluated the copy number variation (CNVs) that may be involved in this process. We found marginally significant SNPs associated with previously described microRNAs, and interesting new non-coding RNAs. Disease resistance genes were also uncovered, probably as a consequence of indirectly selecting resistant trees, although a possible interaction between resistance and heteroblasty cannot be disregarded either. Our work shows the utility and limitations of XP-GWAS analysis to explore the genetic basis of Eucalyptus. © 2023 Elsevier B.V. MenosABSTRACT.- Eucalyptus is one of the mainstays of the forest industry, contributing high-quality raw materials for pulp, paper, wood, and energy production. The typical approaches to reveal the genetic basis of important traits include classical Quantitative Trait Locus (QTL) mapping and Genome-Wide Association Studies (GWAS) approaches, but these are typically expensive and time-consuming. Here we evaluate the potential of Extreme-Phenotype GWAS (XP-GWAS) to identify candidate genes underlying a quantitative trait in Eucalyptus, using the timing of leaf heteroblasty as a case study. XP-GWAS involves genotyping pools of individuals grouped by extreme and opposed phenotypes from a population or a diversity panel and studying their allele frequency. Using a previous phenotyped trial of E. globulus, we sequenced pools of 50 individuals that notably differ in the onset of adult foliage. Since the genetic basis of heteroblasty is well understood, we first searched for previously identified genes. Secondly, we searched for new candidate genes and also evaluated the copy number variation (CNVs) that may be involved in this process. We found marginally significant SNPs associated with previously described microRNAs, and interesting new non-coding RNAs. Disease resistance genes were also uncovered, probably as a consequence of indirectly selecting resistant trees, although a possible interaction between resistance and heteroblasty cannot be disregarded either. Our work shows the utili... Presentar Todo |
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Palabras claves : |
Heteroblasty; MiR156; SISTEMA FORESTAL - INIA; XP-GWAS. |
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Thesagro : |
EUCALYPTUS GLOBULUS. |
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Asunto categoría : |
K01 Ciencias forestales - Aspectos generales |
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Marc : |
LEADER 02909naa a2200265 a 4500
001 1064310
005 2023-09-14
008 2023 bl uuuu u00u1 u #d
022 $a2352-4073
024 7 $a10.1016/j.plgene.2023.100430$2DOI
100 1 $aGIORELLO, F.M.
245 $aEvaluating the potential of XP-GWAS in Eucalyptus$bLeaf heteroblasty as a case study.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 3 February 2023, Revised 24 July 2023, Accepted 11 August 2023, Available online 22 August 2023, Version of Record 31 August 2023. -- Correspondence: Giorello, F.M.; Espacio de Biología Vegetal del Noreste, Centro Universitario Regional Noreste, sede Tacuarembó, Universidad de la República, Tacuarembó, Uruguay; email:facundo.giorello@cut.edu.uy -- FUNDING: This work was supported by grant of the Ministerio de Educación y Cultura, Uruguay (Fondo Vaz Ferreira 173, DICyT, MEC). --
520 $aABSTRACT.- Eucalyptus is one of the mainstays of the forest industry, contributing high-quality raw materials for pulp, paper, wood, and energy production. The typical approaches to reveal the genetic basis of important traits include classical Quantitative Trait Locus (QTL) mapping and Genome-Wide Association Studies (GWAS) approaches, but these are typically expensive and time-consuming. Here we evaluate the potential of Extreme-Phenotype GWAS (XP-GWAS) to identify candidate genes underlying a quantitative trait in Eucalyptus, using the timing of leaf heteroblasty as a case study. XP-GWAS involves genotyping pools of individuals grouped by extreme and opposed phenotypes from a population or a diversity panel and studying their allele frequency. Using a previous phenotyped trial of E. globulus, we sequenced pools of 50 individuals that notably differ in the onset of adult foliage. Since the genetic basis of heteroblasty is well understood, we first searched for previously identified genes. Secondly, we searched for new candidate genes and also evaluated the copy number variation (CNVs) that may be involved in this process. We found marginally significant SNPs associated with previously described microRNAs, and interesting new non-coding RNAs. Disease resistance genes were also uncovered, probably as a consequence of indirectly selecting resistant trees, although a possible interaction between resistance and heteroblasty cannot be disregarded either. Our work shows the utility and limitations of XP-GWAS analysis to explore the genetic basis of Eucalyptus. © 2023 Elsevier B.V.
650 $aEUCALYPTUS GLOBULUS
653 $aHeteroblasty
653 $aMiR156
653 $aSISTEMA FORESTAL - INIA
653 $aXP-GWAS
700 1 $aFARIAS, J.
700 1 $aBASILE, P.
700 1 $aBALMELLI, G.
700 1 $aDA SILVA, C.C.
773 $tPlant Gene. December 2023, Volume 36, article 100430. https://doi.org/10.1016/j.plgene.2023.100430
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
11/05/2021 |
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Actualizado : |
11/05/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
DE SOUZA TEIXEIRA, O.; KUCZYNSKI DA ROCHA, M; MENDES PAIZANO ALFORMA, A.; SILVA FERNANDES, V.; DE OLIVEIRA FEIJÓ, J; NUNES CORRÊA, M.; CANOZZI, M.E.A.; MCMANUS,C.; BARCELLOS, J,O.J. |
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Afiliación : |
ODILENE DE SOUZA TEIXEIRA, Beef Cattle Center for Research on Systems of Beef Cattle Production and Supply Chain, Department of Animal Production, Federal University of Rio Grande do Sul, 91540-000, Porto Alegre, RS, Brazil; MARCELA KUCZYNSKI DA ROCHA, Beef Cattle Center for Research on Systems of Beef Cattle Production and Supply Chain, Department of Animal Production, Federal University of Rio Grande do Sul, 91540-000, Porto Alegre, RS, Brazil.; ANTÓNIA MENDES PAIZANO ALFORMA, Beef Cattle Center for Research on Systems of Beef Cattle Production and Supply Chain, Department of Animal Production, Federal University of Rio Grande do Sul, 91540-000, Porto Alegre, RS, Brazil.; VANESSA SILVA FERNANDES, Beef Cattle Center for Research on Systems of Beef Cattle Production and Supply Chain, Department of Animal Production, Federal University of Rio Grande do Sul, 91540-000, Porto Alegre, RS, Brazil.; JOSIANE DE OLIVEIRA FEIJÓ, Department of Veterinary Clinics, Federal University of Pelotas, 96160-000, Pelotas, RS, Brazil.; MARCIO NUNES CORRÊA, Department of Veterinary Clinics, Federal University of Pelotas, 96160-000, Pelotas, RS, Brazil.; MARÍA EUGENIA ANDRIGHETTO CANOZZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CONCEPTA MCMANUS, Institute of Biological Sciences, University of Brasilia, 70910-900, Brasília, DF, Brazil.; JÚLIO OTÁVIO JARDIM BARCELLOS, Beef Cattle Center for Research on Systems of Beef Cattle Production and Supply Chain, Department of Animal Production, Federal University of Rio Grande do Sul, 91540-000, Porto Alegre, RS, Brazil. |
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Título : |
Behavioural and physiological responses of male and female beef cattle to weaning at 30, 75 or 180 days of age. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Applied Animal Behaviour Science, Volume 240, July 2021, 105339. Doi: https://doi.org/10.1016/j.applanim.2021.105339 |
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DOI : |
10.1016/j.applanim.2021.105339 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT:
Weaning calves at a young age can generate great stress, but it is widely practiced in the industry despite that. So, to what extend weaning in early ages is more stressful than at later ones, in terms of the amount of stress, is currently unknown. Thus, we studied the behavioural and physiological impacts in beef calves at three ages at weaning (30, 75, and 180 days) and the consequences of the calf?s sex. Thirty-six calves were weaned and distributed in hyper-early (W30), early (W75), and conventional (W180) weaning groups, which are weaning
ages that occur in practice. Behavioural activities and physiological parameters were measured on days 0, 1, 2, and 7 after weaning. Canonical component analysis (CCA) and analysis of variance (ANOVA) were used to evaluate behavioural and physiological parameters. The variables analysed were influenced by the treatment, day of evaluation and interaction between treatment × day, and without effect for sex. The younger the calf, the greater the intensity of responses to the weaning stress. In this sense, the behavioural results (percentages of
observations ± SEM) demonstrate that on D0, W30 calves emitted more extreme vocalization (61 %) compared to W75 (15 %) and W180 (0%) (P < 0.001); on D1, W30 and W75 calves expressed more extreme sounds (61 % and 50 %, respectively) than W180 (21 %) (P = 0.028). Similarly, W30 and W75 calves showed a higher number of cross-sucking (P = 0.006). On D0, differences in the frequency of walking (P < 0.001) were observed, once calves of W30 (45.5 ± 6.21 %) and W75 (39.9 ± 6.03 %) spent more time in this activity than W180 ones (17.3 ± 3.80 %). Also, more visiting the feeder without eating feed events were verified (P = 0.014) on D0 (11.5 ± 2.59 %) and D1 (6.2 ± 1.65 %) for W30 when compared to W180 calves (2.6 ± 1.73 % and 0.0 ± 0.00 %, respectively). In physiological terms, a higher respiratory rate (P = 0.043) on D0 was identified for W30 calves (66.3 ± 2.90 breaths/min) than for W75 (57.8 ± 2.30 breaths/min) or W180 (47.3 ± 2.02 breaths/min). Cortisol concentrations were higher (P = 0.019) on D0 for W30 animals (15.6 ± 3.40 ng/mL) than for W180 ones (9.7 ± 1.90 ng/mL). The peak of cortisol occurred on D1 for W30 and D2 for W75 and W180. Therefore, behavioural and physiological changes at young ages indicate greater calf suffering, which may have negative consequences on a productive life. MenosABSTRACT:
Weaning calves at a young age can generate great stress, but it is widely practiced in the industry despite that. So, to what extend weaning in early ages is more stressful than at later ones, in terms of the amount of stress, is currently unknown. Thus, we studied the behavioural and physiological impacts in beef calves at three ages at weaning (30, 75, and 180 days) and the consequences of the calf?s sex. Thirty-six calves were weaned and distributed in hyper-early (W30), early (W75), and conventional (W180) weaning groups, which are weaning
ages that occur in practice. Behavioural activities and physiological parameters were measured on days 0, 1, 2, and 7 after weaning. Canonical component analysis (CCA) and analysis of variance (ANOVA) were used to evaluate behavioural and physiological parameters. The variables analysed were influenced by the treatment, day of evaluation and interaction between treatment × day, and without effect for sex. The younger the calf, the greater the intensity of responses to the weaning stress. In this sense, the behavioural results (percentages of
observations ± SEM) demonstrate that on D0, W30 calves emitted more extreme vocalization (61 %) compared to W75 (15 %) and W180 (0%) (P < 0.001); on D1, W30 and W75 calves expressed more extreme sounds (61 % and 50 %, respectively) than W180 (21 %) (P = 0.028). Similarly, W30 and W75 calves showed a higher number of cross-sucking (P = 0.006). On D0, differences in the frequency of walki... Presentar Todo |
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Palabras claves : |
Animal welfare; Beef cattle; Cortisol Stress; Maternal separation; Walking. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03397naa a2200289 a 4500
001 1062069
005 2021-05-11
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1016/j.applanim.2021.105339$2DOI
100 1 $aDE SOUZA TEIXEIRA, O.
245 $aBehavioural and physiological responses of male and female beef cattle to weaning at 30, 75 or 180 days of age.$h[electronic resource]
260 $c2021
520 $aABSTRACT: Weaning calves at a young age can generate great stress, but it is widely practiced in the industry despite that. So, to what extend weaning in early ages is more stressful than at later ones, in terms of the amount of stress, is currently unknown. Thus, we studied the behavioural and physiological impacts in beef calves at three ages at weaning (30, 75, and 180 days) and the consequences of the calf?s sex. Thirty-six calves were weaned and distributed in hyper-early (W30), early (W75), and conventional (W180) weaning groups, which are weaning ages that occur in practice. Behavioural activities and physiological parameters were measured on days 0, 1, 2, and 7 after weaning. Canonical component analysis (CCA) and analysis of variance (ANOVA) were used to evaluate behavioural and physiological parameters. The variables analysed were influenced by the treatment, day of evaluation and interaction between treatment × day, and without effect for sex. The younger the calf, the greater the intensity of responses to the weaning stress. In this sense, the behavioural results (percentages of observations ± SEM) demonstrate that on D0, W30 calves emitted more extreme vocalization (61 %) compared to W75 (15 %) and W180 (0%) (P < 0.001); on D1, W30 and W75 calves expressed more extreme sounds (61 % and 50 %, respectively) than W180 (21 %) (P = 0.028). Similarly, W30 and W75 calves showed a higher number of cross-sucking (P = 0.006). On D0, differences in the frequency of walking (P < 0.001) were observed, once calves of W30 (45.5 ± 6.21 %) and W75 (39.9 ± 6.03 %) spent more time in this activity than W180 ones (17.3 ± 3.80 %). Also, more visiting the feeder without eating feed events were verified (P = 0.014) on D0 (11.5 ± 2.59 %) and D1 (6.2 ± 1.65 %) for W30 when compared to W180 calves (2.6 ± 1.73 % and 0.0 ± 0.00 %, respectively). In physiological terms, a higher respiratory rate (P = 0.043) on D0 was identified for W30 calves (66.3 ± 2.90 breaths/min) than for W75 (57.8 ± 2.30 breaths/min) or W180 (47.3 ± 2.02 breaths/min). Cortisol concentrations were higher (P = 0.019) on D0 for W30 animals (15.6 ± 3.40 ng/mL) than for W180 ones (9.7 ± 1.90 ng/mL). The peak of cortisol occurred on D1 for W30 and D2 for W75 and W180. Therefore, behavioural and physiological changes at young ages indicate greater calf suffering, which may have negative consequences on a productive life.
653 $aAnimal welfare
653 $aBeef cattle
653 $aCortisol Stress
653 $aMaternal separation
653 $aWalking
700 1 $aKUCZYNSKI DA ROCHA, M
700 1 $aMENDES PAIZANO ALFORMA, A.
700 1 $aSILVA FERNANDES, V.
700 1 $aDE OLIVEIRA FEIJÓ, J
700 1 $aNUNES CORRÊA, M.
700 1 $aCANOZZI, M.E.A.
700 1 $aMCMANUS,C.
700 1 $aBARCELLOS, J,O.J.
773 $tApplied Animal Behaviour Science, Volume 240, July 2021, 105339. Doi: https://doi.org/10.1016/j.applanim.2021.105339
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